Description

Endomark® H1 (LIDM-1201) is a sandwich type immunoassay ready to use allowing to analyse 40 samples in duplicate.

It only requires 100 µl of human plasma to perform this immunoassay.

Blood sampling at admission, renewable for follow-up.

EndoMark®H1 (LIDM-1201) contains all necessary compounds to allow the quantification of endocan in human plasma :

• 6x16 precoated wells with capture antibody
• One vial of anti-endocan monoclonal antibody coupled to biotin, detection antibody
• 5 calibrators
• One positive control
• A bottle of revealing buffer
• A bottle of ELISA buffer 20X
• A bottle of substrate solution
• A bottle of acid stop solution
• Three ELISA adhesives sealing films
• Instructions of use

Characteristics

• Linearity : 0.3 à 5 ng/ml
• Detection limit : 0.15 ng/ml
• Quantification limit : 0.3 ng/ml
• Intra-series repeatability (%CV) : 4,80%
• Inter-series reproductibility (%CV) : 7,59%
• Interferences: no interferences observed with hemolysed, lactescent or icteric plasma

Documentation

• Instructions of use

• Madhivathanan PR, Fletcher N, Gaze D, Thomson R, Chandrasekaran V, Al-Subaie N, Valencia O, Sharma V. Perioperative kinetics of endocan in patients undergoing cardiac surgery with and without cardiopulmonary bypass. Cytokine. 2016 Mar 18;83:8-12.

Check our pages Clinical areas and Endocan for more information.