Description

• Solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors
• Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations are determined by Lowry method
• Endotoxin units are measured by Limulus Amoebocyte Lysate assay
• Gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay (Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hrs without addition of nerve growth factor)