Description

A sterile, concentrated (100X) solution intended for use with Medium 254 or Medium 254CF to culture human epidermal melanocytes. Each 5 ml bottle of HMGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal melanocytes and is the correct amount of supplement for a 500 ml bottle of basal Medium 254 or Medium 254CF.

When a 500 ml bottle of medium is supplemented with HMGS, the final concentrations of the components in the supplemented medium are:

Bovine pituitary extract (BPE) (0.2% v/v)

Fetal bovine serum (0.5% v/v)

Recombinant human insulin-like growth factor-I (1µg/ml)

Bovine transferrin (5µg/ml)

Basic fibroblast growth factor (3 ng/ml)

Hydrocortisone (0.18µg/ml)

Heparin (3µg/ml)

Phorbol 12-myristate 13-acetate (PMA) (10 ng/ml)

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