Description

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon . It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.

Ready-to-use 100mM IPTG aqueous solution is non-toxic, stable, 0.22µm membrane-filtered, formulated for direct use in blue/white colony screening.