Description

The 5' RACE System provides a set of prequalified reagents intended for synthesis of first-strand cDNA, purification of first-strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR. Control RNA, DNA, and primers are provided for monitoring system performance. The system is useful for the amplification of rare messages for which little sequence information may be available, and for capturing the 5' end information of mRNA.

• Uses advanced capabilities of SuperScriptII RNase H- RT for greater first-strand cDNA yields and increased cDNA lengths
• Prepares specific cDNA from up to 1µg of total or poly(A)+ RNA in one reaction
• Offers improved first-strand cDNA purification procedure that yields cDNA suitable for tailing
• Uses recombinant TdT with optimized reaction conditions in PCR-compatible buffer to provide efficient primer annealing site for PCR

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

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