Description

The GeneRacer Kit provides a method to obtain full-length 5' and 3' ends of cDNA using known cDNA sequence from expressed sequence tags (ESTs), subtracted cDNA, differential display, or library screening. RACE PCR products can be quickly and easily cloned using either the Zero Blunt TOPO PCR Cloning Kit for Sequencing (blunt-end PCR products) or the TOPO TA Cloning for Sequencing Kit (PCR products with 3' A-overhangs). Using the protocols provided, the cDNA ends of rare (30 copies/cell) and long (9 kb) transcripts can be amplified and sequenced starting from 1µg of total RNA.

• Generate cDNA from transcripts at least 10kb in length
• Obtain full-length 5' end of rare transcripts at fewer than 30 copies per cell
• Clone full-length 5' and 3' ends to construct complete cDNA sequence

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

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