Description

• Tested for the ability to promote neurite outgrowth of chick dorsal root ganglia cells
• Tested and found negative for bacteria, fungi, and mycoplasma
• Source: Engelbreth-Holm-Swarm (EHS) mouse tumor, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors
• Base formulation: Dulbecco's Modified Eagle's Medium with 50µg/mL gentamycin, with or without Phenol Red
• Solubilized basement membrane preparations polymerize at room temperature

Quality

• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens
• Tested and found negative for bacteria, fungi and mycoplasma
• Protein concentrations determined by Lowry method
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay

Application

• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• In Vitro and In Vivo Angiogenesis Assays
• In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice