Description

• Ideal for first- and second-strand synthesis of cDNA, primer extensions and RNA sequencing, preparation of labeled hybridization probes, and RT-PCR
• Well suited for templates with high secondary structures due to its stability at higher reaction temperatures (37° to 58°C)
• Requires a primer and Mg²⁺ or Mn²⁺ for activity
• Possesses an intrinsic RNase H activity, but is free of contaminating DNases and RNases
• Stabilized in vitro by nonionic detergents and sulfhydryl compounds

Provided buffer is not compatible with RT-PCR; for RT-PCR, Reverse Transcription 10X Buffer (Mfr. No. A3561) should be used in combination with MgCl₂ (Mfr. No. A3511).