Description

• Offers robust amplification equal to—and in some cases superior to—conventional Taq DNA polymerase
• Suitable for PCR, two-step RT-PCR, and T-vector cloning
• Utilizes a separate MgCl₂ solution to allow for optimization of magnesium concentration
• Samples can be loaded directly onto a gel after amplification
• Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl₂, 200µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10µg activated calf thymus DNA and 0.1mg/mL BSA in a final volume of 50µL.