Description

DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq buffer, which includes 20 mM MgCl₂.

DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl_2, and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

DreamTaq DNA Polymerase ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq buffer, which includes 20 mM MgCl₂. DreamTaq DNA Polymerase generates PCR products with 3'-dA overhangs. It does not incorporate dUTP.

• Robust amplification with minimal optimization
• High yields of PCR products
• Higher sensitivity compared to conventional Taq DNA polymerase
• Amplification of long targets up to 6kb from genomic DNA and up to 20kb from viral DNA
• Generates 3'-dA overhangs
• Incorporates modified nucleotides

Definition of Activity Unit

• One unit of the enzyme catalyzes the incorporation of 10nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C
• Enzyme activity is assayed in the following mixture: 67mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl₂, 1mM 2-mercaptoethanol, 50mM NaCl, 0.1mg/mL BSA, 0.75mM activated calf thymus DNA, 0.2mM of each dNTP, 0.4MBq/mL [3H]-dTTP

Storage Buffer

• The enzyme is supplied in: 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, stabilizing agent and 50% (v/v) glycerol

Quality Control

• The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests; Functionally tested in PCR

Inhibition and Inactivation

• Inhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1)
• Inactivated by phenol/chloroform extraction

Recommended for:

Routine PCR amplification of DNA fragments up to 6kb; RT-PCR; Genotyping; Generation of PCR products for TA cloning

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