Description

RNA targets from 100 bp to >12kb can be detected with this system. The amount of starting material can vary from 0.1pg to 5µg of total RNA.

• Kit includes SuperScript™ III/RNaseOUT™ Enzyme Mix, 2X First-Strand Reaction Mix, and Annealing Buffer
• SuperScript™ III Reverse Transcriptase, included in the Enzyme Mix, is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
• Enzyme can be used to synthesize cDNA at a temperature range of 45° to 55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases
• Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA
• RNaseOUT™ Recombinant RNase Inhibitor is included in the Enzyme Mix to safeguard against degradation of target RNA due to ribonuclease contamination
• 2X First-Strand Reaction Mix includes 10mM MgCl₂ and 1mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA
• Annealing Buffer is used in the initial template-primer annealing step
• Separate tubes of oligo(dT)20 and random hexamers are also provided. cDNA synthesis can be performed using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer

• Optimal reaction temperature: 50°C
• Enough material provided for 50 reactions (20µL each)

PCR & Real-Time PCR, Reverse Transcription

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