Description

Incorporating a unique dsDNA–binding domain, Phusion DNA Polymerase amplifies DNA with accuracy and speed unattainable with standard PCR enzymes, even on the most difficult templates. Featuring an error rate 50–fold lower than that of Taq, and 6–fold lower than that of Pfu, Phusion DNA Polymerase is the most accurate thermostable polymerase available. This feature makes Phusion DNA Polymerases superior choice for cloning and other applications requiring high fidelity. The unique structure of the enzyme enables short protocol times, abundant yields and robust performance, even in the presence of PCR inhibitors. For the researcher, that translates to ease–of–use and convenience. In addition, Phusion DNA Polymerases also produce higher yields with lower enzyme amounts than other DNA polymerases.

Thermo Scientific Phusion DNA Polymerases set a gold standard for PCR performance. They offer a combination of characteristics that no other enzyme can match. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion DNA Polymerase is the most accurate thermostable polymerase available. This feature makes Phusion DNA Polymerases superior choice for cloning and other applications requiring high fidelity. The unique structure of the enzyme enables short protocol times, abundant yields and robust performance, even in the presence of PCR inhibitors. For the researcher, that translates to ease-of-use and convenience. In addition, Phusion DNA Polymerases also produce higher yields with lower enzyme amounts than other DNA polymerases.
New Phusion Green format is a combination of Phusion DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with excelent performance of Phusion DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Phusion High-Fidelity PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. Only template and primers need to be added by the user. Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control template and primers.


Highlights

• Extreme fidelity (error rate 4.4 x 10^-7 in Phusion HF Buffer)
• Polymerase speed allows short extension times (15 to 30 s/kb)
• Robust performance, minimal optimization needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gel with Green Buffer

Applications

• High performance PCR
• High-fidelity PCR
• Cloning
• Sequencing (template generation)
• High throughput
• Difficult (GC-rich) templates
• Template generation for sequencing
• Long-range PCR
• Cloning
• Mutagenesis†
• Microarray

Includes
Phusion High-Fidelity DNA Polymerase (F-530):

• Phusion DNA Polymerase 2 U/µL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl₂ solution
  Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl₂ in the final 1X concentration.

Phusion Green High-Fidelity DNA Polymerase (F-534):

• Phusion DNA Polymerase 2 U/µL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl₂
  Both Phusion Green HF and Phusion Green GC Buffer provide 1.5 mM MgCl₂ in the final 1X concentration.

Phusion High-Fidelity PCR Master Mix with HF Buffer (F-531):

• Phusion DNA Polymerase, 400 µM of each dNTP and 2X Phusion HF Buffer
• DMSO

Phusion High-Fidelity PCR Master Mix with GC Buffer (F-532):

• Phusion DNA Polymerase, 400 µM of each dNTP and 2X Phusion GC Buffer
• DMSO

Master mixes provide 1.5 mM MgCl₂ and 200 µM dNTP in final reaction concentration.

Phusion High-Fidelity PCR Kit (F-553):

• Phusion DNA Polymerase 2 U/µl
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl₂ solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 µM each)
• 10 kb primers (4 µM each)
• DNA size standard
• DMSO

Hazardous: No
Shelf Life: 0 Months,36 Months
Shipping Condition: Dry Ice
Storage buffer: 20 mM Tris-HCl (pH 7.4 at 25 °C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/mL BSA and 50 % glycerol.
Storage Condition: -20°C