Description

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.
In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start . These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.
Phusion U Hot Start DNA Polymerase is also available in the Master Mix and Green formats. Phusion U Hot Start PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. Only template and primers need to be added by the user. Phusion U Green format is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Highlights
·Accuracy - extremely high fidelity DNA polymerase (25x Taq)
·Uracil-tolerance - engineered to incorporate dUTP and amplify uracil containing templates
·Specificity - hot start for reduced nonspecific amplification and primer degradation.
·Speed - short extension times (15-30 s/kb)
· Direct loading of PCR products on gels
Applications
· Amplification of bisulphite-converted DNA
· Amplification of damaged or aged DNA
· Carry-over contamination control
· Uracil-excision based (USER) cloning methods
Includes
Phusion U Hot Start DNA Polymerase (F-555)
· Phusion U Hot Start DNA Polymerase, 2 U/µL
· 5X Phusion HF Buffer
· 5X Phusion GC Buffer
· DMSO
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl₂ in the final 1X concentration.
Phusion U Green Hot Start DNA Polymerase (F-556)
· Phusion U Hot Start DNA Polymerase, 2 U/µL
· 5X Phusion Green HF Buffer
· 5X Phusion Green GC Buffer
· DMSO
Both Phusion Green HF and Phusion Green GC Buffer provide 1.5 mM MgCl₂ in the final 1X concentration.
Phusion U Hot Start PCR Master Mix
· 2X Phusion U PCR Master Mix (Phusion U Hot Start DNA Polymerase, 2X reaction buffer, 3 mM MgCl₂, 400 µM of each dATP, dTTP, dCTP, and dGTP)
· Water, nuclease-free

Hazardous:No
> Storage buffer:20 mM Tris-HCl (pH 7.4 at 25°C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, and 50 % glycerol.
> Storage Condition:-20°C

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