Description

Phire Hot Start II DNA Polymerase is a novel PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

The hot start modification of Phire Hot Start II DNA Polymerase is based on the same Affibody inactivation technology utilized by Phusion Hot Start II DNA Polymerase. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.

New Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Phire Hot Start II DNA Polymerase is an upgraded version of the original Phire Hot Start DNA Polymerase. Due to improved Affibody-based hot start technology the polymerase has no annealing temperature limitations and it is extremely fast (extension time of only 10-15 s/kb). The unique dsDNA-binding domain makes Phire Hot Start II DNA Polymerase very robust and capable of amplifying long DNA fragments with high yields. The Affibody-based inactivation method of Phire Hot Start DNA Polymerase increases the specificity of the amplification at ambient temperatures but allows immediate and complete reactivation of the enzyme at standard cycling temperatures.

• Quick hot start: No reactivation step
• Fast enzyme: Amplify four to five times faster than with hot start Taq
• Robust: Minimal reaction optimization due to high inhibitor tolerance
• High yields: Abundant products due to high efficiency
• Longer PCR products: Amplify significantly longer DNA fragments than with any hot start Taq
• No annealing temperature limitations

Includes:

Phire Hot Start II DNA Polymerase is supplied with 5x Phire Reaction Buffer and DMSO. The buffer provides 1.5 mM MgCl₂ in the final 1x concentration.

Recommended for:

Fast PCR, routine PCR, high-throughput PCR, long-range PCR, DHPLC, microarray

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