Description

The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR is a convenient system optimized for cDNA synthesis in 2-step quantitative RT-PCR (RT-qPCR) applications. The kit uses Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT.
The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 µg) at elevated temperatures (42 to 65°C).The synthesis reaction can be completed in 15 to 30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.
The new Maxima First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

Highlights

• Integrated gDNA removal step
• High yields of full length cDNA up to 20 kb.
• Efficient cDNA synthesis at wide temperature range from 42°C to 65°C.
• Increased synthesis rate - complete cDNA synthesis in 15-30 minutes.
• High sensitivity and specificity.

Applications

• 2-step RT-PCR
• 2-step RT-qPCR

Includes
Maxima First Strand cDNA Synthesis Kit for RT-qPCR contains

• Maxima Enzyme Mix (Maxima Reverse Transcriptase and RiboLock RNase Inhibitor)
• 5X Reaction Mix (reaction buffer, dNTPs, oligo (dT)₁₈ and random hexamer primers)
• nuclease-free water

Maxima First Strand cDNA Synthesis Kit with dsDNase contains

• Maxima Enzyme Mix (Maxima Reverse Transcriptase and RiboLock RNase Inhibitor)
• 5X Reaction Mix (reaction buffer, dNTPs, oligo (dT)18 and random hexamer primers)
• dsDNase
  · 10X dsDNase Buffer
• nuclease-free water

Additional Information about Reaction Components
The recombinant RiboLock RNase Inhibitor, included in Maxima Enzyme Mix, effectively protects RNA templates from degradation by RNases A, B and C at temperatures up to 55°C.

Oligo(dT)₁₈ and random hexamer primers are supplied with the kit. Random hexamer primers bind non-specifically and are used to synthesize cDNA from all RNAs in a total RNA population. The oligo(dT)₁₈ primer selectively anneals to the 3'-end of poly(A) RNA, synthesizing cDNA only from poly(A)-tailed mRNA. Gene-specific primers may also be used with the kit to prime synthesis from a specified sequence.

Water, nuclease-free is provided for reaction set-up and dilution of sample RNA. The absence of endo-, exodeoxyribonucleases, ribonucleases and phosphatases has been confirmed by appropriate quality tests.

Maxima Universal First Strand cDNA Synthesis Kit
Only available in US and Canada

The Maxima Universal First Strand cDNA Synthesis Kit differs from the Maxima First Strand cDNA Synthesis Kit by having separate kit c

Hazardous: No
Quality Control:

• Functionally tested in two-step RT-qPCR using different starting amounts (5 ng to 0.5 fg) of RNA transcript in reverse Transcription reactions followed by amplification with Maxima SYBR Green/ROX qPCR Master Mix.
• Efficiency is in the range of 90 to 110%, the slope is between -3.09 and -3.58 with a correlation coefficient > 0.99.

Shipping Condition: Dry Ice
Storage Condition: -20°C

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