Description

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized reagents, sampling tools, and DNARelease Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes detailed instructions and control primers that can be used with numerous animal species.
Animal Direct PCR Protocol
Highlights
· No need for time-consuming and expensive DNA purification steps
· Very little sample material required
· Two simple protocols for various applications
· Convenient sampling tools included in the kit
·Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).
Includes
· Phire Hot Start II DNA Polymerase
·Reaction voume with dilution & storage protocol: 20 µL
·Reaction voume with direct protocol: 50 µL
· 2X Phire Animal Tissue PCR Buffer (includes dNTPs and MgCl₂)
· Universal control primer mix
· Dilution Buffer
· DNARelease Additive
· Gel loading dye
Two alternative protocols suitable for various purposes
In the direct protocol, a small piece of animal tissue is placed directly into the PCR reaction. In the dilution protocol, a small amount of animal tissue is first briefly incubated in incubation buffer and 1 µL of this buffer is then added into the PCR reaction. The dilution protocol is useful if several PCR reactions are performed from the same sample.

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