Description

Transposon technology is the first technology that allows scientist to insert constructed linear DNA fragment into circular or linear DNA target randomly and very efficiently. The Thermo Scientific Template Generation System II Kit (TGS II Kit) is based on the transposition reaction of the bacteriophage Mu. This system has been simplified to work in vitro, and an artificial Mu transposon, designated as the Entranceposon, has been constructed. The reaction is catalyzed by a single enzyme, MuA Transposase.

TGS II Kit provides the tools for inserting the Entranceposon into foreign DNA at random locations. Insertion of an Entranceposon into unknown target DNA provides primer binding sites for different applications. A simple and fast in vitro transposition reaction takes place in a single reaction tube.

Highlights

• Provides templates for DNA sequencing more simply and with less hands-on time than any other method
• Thousands of ready-to-sequence templates from a single transposition reaction: Enough to sequence even large DNA clones (e.g. BAC clones) without fragmentation or subcloning
• Simple mapping of the Entranceposon insertions by colony-PCR or restriction enzyme digestion enables directed sequencing. Fewer sequencing reactions are required to complete a sequence than with the shotgun approach
• Universal primers: The sequencing and mapping primers are included in the kit, no need for custom primers.
• Bidirectional sequencing: A single template clone can be used for sequencing the flanking DNA on both sides of the Entranceposon insertion
• Three different antibiotic resistance genes are available in each TGS II Kit

All components needed as well as detailed instruction manual to perform the reactions are included in the kit. TGS II Kit is an updated version of the TGS I Kit.