Description

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material.
Phire Plant Direct PCR Kit has been tested with leaves and seeds from a wide variety of plant species. The kit includes a complete set of optimized reagents, sampling tools and detailed protocols making it an ideal choice for amplification of plant DNA.When combined with Piko Thermal Cycler and Piko UTW PCR Plates, PCR protocols can be completed in as little as 30 minutes.
Highlights
· No need for time-consuming and expensive DNA purification steps
· Very little sample material required
· Two simple protocols for various applications
· Convenient sampling tools included in the kit
·Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/ kb).
Applications
· Genotyping
· Transgene detection
Includes
· Phire Hot Start II DNA Polymerase
· Reaction voume with dilution & storage protocol: 20 µL
· Reaction voume with direct protocol: 50 µL
· 2X Phire Plant PCR Buffer (includes dNTPs and MgCl₂)
· Control Primer Mix (25 µM each)
· Dilution Buffer
Two short Protocols for various needs
In the direct protocol, a small piece of plant material is placed directly in the PCR reaction mix. In the dilution protocol, a small volume of the Dilution Buffer containing crushed plant material is added in the PCR reaction mix. The dilution protocol is used for long or difficult amplicons, and for assays where several DNA fragments are amplified from the same sample.