Description

SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can utilize either blue light or UV excitation.

• Reduces exposure to highly mutagenic ethidium bromide and harmful UV light
• Increases sensitivity by reducing nonspecific background fluorescence
• Use in place of ethidium bromide for all staining applications, including RNA staining
• Less hazardous

A better DNA stain SYBR Safe DNA Gel Stain is a better nucleic acid staining reagent for molecular biology applications. Safer than ethidium bromide SYBR Safe DNA Gel Stain has been specifically formulated and evaluated in a battery of toxicity tests with results indicating that it is less mutagenic and safer than ethidium bromide. An independent laboratory showed reduced mutagenicity of SYBR Safe stain compared to ethidium bromide using the Ames test. Further decrease exposure risk by using visible blue-light illumination with SYBR Safe stain rather than UV illumination, especially when performing exposure-intensive protocols like cutting bands out of gels.
Excellent sensitivity SYBR Safe stain offers excellent sensitivity in nucleic acid visualization and documentation applications with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation maxima at ~280 and ~502nm, and an emission maximum at ~530nm. Plus, when used with blue light illumination, SYBR Safe stain has less background fluorescence than ethidium bromide–stained gels illuminated with UV light.
Easy to use SYBR Safe stain is supplied as a 10,000X concentrate in DMSO which can be used like a solution of ethidium bromide. SYBR Safe stain can also be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of SYBR Safe stain following electrophoresis.

Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting

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