Description

• In the first step, live cells can be 'fixed' with the Fixation Buffer, which cross- links proteins
• The second step, using the Permeabilization Buffer, creates holes in the membrane and allows the intracellular staining antibodies to enter the cell effectively
• Subsequent washing steps, antibody additions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer
• Final washing step and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer (cat. 00-4222).
• This kit is commonly used to stain cytoplasmic proteins and proteins in the secretory pathway such as chemokines and cytokines
• Not recommended for intranuclear staining. Instead, please refer to the Foxp3/Transcription Factor Buffer Set for nuclear staining
• Before the fixation step, it is recommended to label the live cells using one of the Fixable Viability Dyes and then proceed with fixation