产品名称 IgG antibody | Biorbyt
产品货号 orb108248
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name IgG antibody
Catalog Number orb108248
Species/HostGoat
ConjugationUnconjugated
Tested applicationsELISA, IHC-Fr, WB
Target IgG. Reacts with the heavy chains on human IgG but not with the light chains on most human immunoglobulins.No antibody was detected against human IgM or IgA, or against non immunoglobulin serum proteins, butantibodies may cross react with immunoglobulins from other species.
Form/Appearance Lyophilized from 0.01M Sodium phosphate, 0.25M Sodium chloride, pH 7.6 containing 15mg/ml BSA
Concentration 0.8mg/ml (prior to lyophilization)
Preservatives No Preservative.
Storage Store lyophilized product at 2-8°C. After reconstitution, product is stable for several weeks at 2-8°C as an undiluted liquid. Prepare working dilution only prior to immediate use. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final concentration of 50% followed by storage at-20°C, with or without aliquotting. Please note that the concentration of protein and buffer salts will decrease of one-half of the original after the addition of glycerol. Expiration date: One year from date of reconstitution.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified
Biological Origin Sample obtained from Goat.
Dilution Range IHC-Fr:1:500-1:5,000, ELISA:1:5,000-1:100,000, WB:1:10,000-1:200,000
Application Notes A dilution range of 1:500-1:5,000 is suggested for immunohistochemistry on tissue sections, 1:5,000-1:100,000 for ELISA and Western blotting with chromogenic substrates and 1:10,000-1:200,000 forWestern blotting with ECL substrates.Each laboratory should determine an optimum working titer for use inits particular application.Other applications have not been tested but use in such assays should notnecessarily be excluded.Use of sodium azide as a preservative will substantially inhibit the enzyme activity ofhorseradish peroxidase.Centrifuge before opening to ensure complete recovery of vial contents.
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