产品名称 IgG antibody | Biorbyt
产品货号 orb108283
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name IgG antibody
Catalog Number orb108283
Species/HostGoat
ConjugationUnconjugated
Tested applicationsELISA, IHC-Fr, WB
Target IgG. Based on immunoelectrophoresis, the antibody reacts with the light chains on human IgG and with thosecommon to other human immunoglobulins. Does not react with the Fc portion of human IgG. No antibodywas detected against non immunoglobulin serum proteins, but antibodies may cross react withimmunoglobulins from other species.
Form/Appearance Lyophilized from 0.01M Sodium Phosphate, 0.25M Sodium Chloride, pH 7.6 containing 15mg/ml BSA
Concentration 0.8mg/ml (prior to lyophilization)
Preservatives No Preservative.
Storage Store lyophilized product at 2-8°C. After reconstitution, product is stable for several weeks at 2-8°C as an undiluted liquid. For long term storage, aliquot and store at-20°C. For extended storage, add an equal volume of glycerol (ACS or better grade) for a final concentration of 50% followed by storage at-20°C. Please note that the concentration of protein and buffer salts will be one-half of the original after the addition of glycerol. In the absence of glycerol, the product may be aliquotd and stored at-70°C or below. Avoid multiple freeze/thaw cycles. Prepare working dilutions only prior to immediate use. Expiration date is one year from date of reconstitution.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified
Biological Origin Sample obtained from Goat.
Dilution Range IHC-Fr:1:500-1:5,000, ELISA:1:5,000-1:100,000, WB:1:10,000-1:200,000
Application Notes A dilution range of 1:500-1:5,000 is suggested for immunohistochemistry on tissue sections, 1:5,000 -1:100,000 for ELISA and Western blotting with chromogenic substrates and 1:10,000-1:200,000 forWestern blotting with ECL substrates.Each laboratory should determine an optimum working titer for use inits particular application.Other applications have not been tested but use in such assays should notnecessarily be excluded.Use of sodium azide as a preservative will substantially inhibit the enzyme activity ofhorseradish peroxidase.Centrifuge before opening to ensure complete recovery of vial contents.
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