产品名称 Sheep Serum Against Human Ig Kappa Light Chains
产品货号 orb22435
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Sheep Serum Against Human Ig Kappa Light Chains
Catalog Number orb22435
Species/HostSheep
ConjugationUnconjugated
Tested applicationsDOT, IEP
Immunogen A pool of purified Bence Jones kappa proteins isolated from human urine. Freunds complete adjuvant is used in the first step of the immunization procedure.
Target Ig Kappa (light chain). The reactivity of the antiserum is directed to the hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is only reacting with Bence Jones proteins as well as free light chains of the kappa type. No reaction is obtained with intact polyclonal or monoclonal immunoglobulins.
Form/Appearance Vial with 1 ml lyophilized antiserum.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled sheep serum. No foreign proteins added.
Preservatives No preservative added, as it may interfere with the antibody activity
Storage The lyophilised product is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles. Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Note For research use only.
ClonalityPolyclonal
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with human serum proteins. Further adsorption by stepwise soluble adsorption to eliminate antibodies reacting with bound kappa chain.
Application Notes Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross- reactivity of this antiserum has not been tested in detail.
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