| 产品名称 | Sheep Serum Against Human Ig Kappa Light Chains |
| 产品货号 | orb21885 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Sheep Serum Against Human Ig Kappa Light Chains |
|---|---|
| Catalog Number | orb21885 |
| Species/Host | Sheep |
| Conjugation | Unconjugated |
| Tested applications | DOT, IEP |
| Target | Ig Kappa (light chain). The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of mouse serum or plasma. |
| Form/Appearance | Vial with 1 ml lyophilized antiserum. |
| Concentration | Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled sheep serum. No foreign proteins added. |
| Preservatives | No preservative added, as it may interfere with the antibody activity |
| Storage | The lyophilised product is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles. Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with mouse serum proteins. |
| Biological Origin | A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
| Application Notes | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. IE: In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. When carrying out analyses of Bence Jones proteins or free light chains in urine or serum using an immunodiffusion technique, special attention should be given to their faster rate of diffusion as compared to that of the antibody molecules in the antiserum. If they both start to diffuse into the gel in a double radial diffusion at the same time, an excess of the antigen may easily prevent visible precipitation. To prevent false negative results, the antiserum should be diffuse for about 2 hours before the antigen reservoir is filled. |
| 产品资料 |