| 产品名称 | Rabbit IgG Against Mouse IgG Fab |
| 产品货号 | orb21670 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Rabbit IgG Against Mouse IgG Fab (TRITC) |
|---|---|
| Catalog Number | orb21670 |
| Species/Host | Rabbit |
| Conjugation | TRITC |
| Tested applications | DOT, IEP |
| Immunogen | Purified Fab of normal IgG isolated form mouse serum. Freunds complete adjuvant is used in the first step of the immunization procedure. |
| Target | Rabbit IgG Against Mouse IgG Fab (TRITC). The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both light chain types, with their Fab or F(ab)2 subunits, and with free light chains of kappa and lambda type. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled mouse serum a characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line of the purified Fab. |
| Form/Appearance | Vial with 1 ml lyophilized immunoconjugate. |
| Concentration | IgG protein concentration 1 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.4. No foreign proteins added. |
| Preservatives | No preservative added as it may interfere with he antibody activity. |
| Storage | The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application Notes | Conjugation Notes: A proprietary technique for the binding to TRITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence. Excitation: 554 nm, emission: 573 nm. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell- counting procedures. Experiment Notes: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography. |
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