| 产品名称 | Goat IgG Against Rat IgG2B |
| 产品货号 | orb21353 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Goat IgG Against Rat IgG2B (TRITC) |
|---|---|
| Catalog Number | orb21353 |
| Species/Host | Goat |
| Conjugation | TRITC |
| Tested applications | DOT, IEP |
| Immunogen | Pools of purified homogenous IgG2b isolated from rat serum. Freunds complete adjuvant is used in the first step of the immunization procedure. |
| Target | Goat IgG Against Rat IgG2B (TRITC). The reactivity of the antiserum is directed to the subclass IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immuno- electrophoresis and double radial immunodiffusion. |
| Form/Appearance | Vial with 1 ml lyophilized immunoconjugate. |
| Concentration | IgG protein concentration 1 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.8. No foreign proteins added. |
| Preservatives | No preservative added, as it may interfere with the antibody activity. |
| Storage | The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application Notes | This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion a weak reaction with mouse has been observed. Conjugation Notes: A proprietary technique for the binding of TRITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence. Excitation: 554 nm, emission: 573 nm. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell-counting procedures. Experiment Notes: Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis. |
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