| 产品名称 | Goat IgG Against Rat IgE |
| 产品货号 | orb22067 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Goat IgG Against Rat IgE (TRITC) |
|---|---|
| Catalog Number | orb22067 |
| Species/Host | Goat |
| Conjugation | TRITC |
| Tested applications | DOT, IEP |
| Immunogen | Purified homogenous IgE isolated from pooled rat serum. Freunds complete adjuvant is used in the first step of the immunization procedure. |
| Target | Goat IgG Against Rat IgE (TRITC). The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule as tested by gel- immunodiffusion techniques against rat sera and a panel of purified homogenous immunoglobulins. A reaction of full identity is obtained with serum containing monoclonal IgE and with the immunogen. No reaction is obtained with normal rat serum. The product does not react with any other protein of rat plasma or serum. |
| Form/Appearance | Vial with 1 ml lyophilized immunoconjugate. |
| Concentration | IgG protein concentration 7.1 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.7. No foreign proteins added. |
| Preservatives | No preservative added |
| Storage | The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other component of the rat immunoglobulin system with special emphasis on the common Fab portion, and to any other serum protein. |
| Application Notes | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Conjugation Notes: A proprietary technique for the binding to TRITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence. Excitation: 554 nm, emission: 573 nm. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell-counting procedures. Experiment Notes: The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion. |
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