| 产品名称 | Sheep IgG Against Rat IgG Fab |
| 产品货号 | orb21660 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Sheep IgG Against Rat IgG Fab (HRP) |
|---|---|
| Catalog Number | orb21660 |
| Species/Host | Sheep |
| Conjugation | HRP |
| Tested applications | DOT, IEP |
| Immunogen | Purified normal Fab of polyclonal IgG isolated from pooled rat serum. Freunds complete adjuvant is used in the first step of the immunization procedure. |
| Target | Sheep IgG Against Rat IgG Fab (HRP). The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both light chain types, with their Fab or F(ab)2 subunits, and with free light chains of kappa and lambda type. In immuno- electrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled rat serum a characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line of the purified Fab. |
| Form/Appearance | Vial with 1 ml lyophilized immunoconjugate. |
| Concentration | IgG protein concentration 1 mg/ml. Peroxidase/IgG protein molar ratio (E/P) is approximately 1.7. No foreign proteins added. |
| Preservatives | No preservative added, as it may interfere with he antibody activity. |
| Storage | The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, no t refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application Notes | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Conjugation Notes: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life. Experiment Notes: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography. |
| 产品资料 |