产品名称 Goat IgG Against Guinea Pig IgG Fab
产品货号 orb21859
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Goat IgG Against Guinea Pig IgG Fab (FITC)
Catalog Number orb21859
Species/HostGoat
ConjugationFITC
Tested applicationsDOT, IEP
Immunogen Purified normal Fab of polyclonal guinea pig IgG. Freunds complete adjuvant is used in the first step of the immunization procedure.
Target Goat IgG Against Guinea Pig IgG Fab (FITC). The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both light chain types, with their Fab or F(ab)2 subunits, and with free light chains of kappa and lambda type. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled guinea pig serum a characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line of the purified Fab.
Form/Appearance Vial with 1 ml lyophilized immunoconjugate.
Preservatives No preservative added as it may interfere with he antibody activity.
Storage The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Note For research use only.
ClonalityPolyclonal
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Notes Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.

Experiment Notes: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
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