| 产品名称 | Sheep IgG Against Mouse Ig Kappa Light Chains |
| 产品货号 | orb21410 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Sheep IgG Against Mouse Ig Kappa Light Chains (FITC) |
|---|---|
| Catalog Number | orb21410 |
| Species/Host | Sheep |
| Conjugation | FITC |
| Tested applications | DOT, IEP |
| Immunogen | A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freunds complete adjuvant is used in the first step of the immunization procedure. |
| Target | Sheep IgG Against Mouse Ig Kappa Light Chains (FITC). The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of mouse serum or plasma. |
| Form/Appearance | Vial with 1 ml immunoconjugate. |
| Concentration | IgG protein concentration is 1 mg/ml. Fluorochrome/IgG protein ratio (F/P) approxi- mately 1.6. No foreign proteins added. |
| Preservatives | No preservative added, as it may interfere with the antibody activity |
| Storage | The lyophilised product is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen , and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other mouse serum proteins. |
| Application Notes | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Conjugation Notes: A proprietary technique for the binding to FITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Fluorescein isothiocyanate isomer 1. Excitation: 492 nm, emission: 515 nm. Experiment Notes: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography. |
| 产品资料 |