产品名称 Hepatitis B Associated antigen antibody
产品货号 orb21249
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Hepatitis B Associated antigen antibody
Catalog Number orb21249
Species/Host Porcine
Reactivity Human
Conjugation Unconjugated
Tested applications DOT, IEP
Immunogen The immunogen has been prepared from sera of patients with hepatitis using gelfiltration Freunds complete adjuvant is used in the first step of the immunization procedure.
Target Hepatitis B Associated antigen. The defined antibody specificity is to the hepatitis associated antigen as tested in counterelectrophoresis and double radial immunodiffusion against HAA positive human serum. The product does not react with normal human serum or plasma.
Form/Appearance Vial with 1 ml lyophilized antiserum.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled swine serum. No foreign proteins added.
Preservatives No preservative added.
Storage The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Note For research use only.
Clonality Polyclonal
Adsorption Immunoaffinity adsorbed using pooled human serum negative for the antigen, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Notes Inter-species cross-reactivity of this antiserum has not been tested in detail.

IE: In counterelectrophoresis use opposite holes at a distance of 4mm with 6 μl antiserum and serum samples. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. The distance between the wells should be 2-3 mm. In case the concentration of the protein component is significantly increased or reduced, the required equivalence between the reactants can be establish by increasing the amount of antiserum or antigen respectively.
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