产品名称 | Mouse Mab Against Human IgG3 |
产品货号 | orb22276 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | |
产品品牌 | Biorbyt |
产品概述 | |
产品详情 | |
Product Name | Mouse Mab Against Human IgG3 (TRITC) |
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Catalog Number | orb22276 |
Species/Host | Mouse |
Conjugation | TRITC |
Tested applications | DOT, IEP |
Immunogen | Highly purified monoclonal IgG3m(u) isolated from human serum. |
Target | Mouse Mab Against Human IgG3 (TRITC). The reactivity of the antiserum is restricted to an allotype specific determinant on the IgG3m(u) molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. |
Form/Appearance | Vial with 0.5 ml lyophilized immunoconjugate. |
Concentration | IgG concentration is 0.4 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) approximately 1.5. No foreign proteins added. |
Preservatives | No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Storage | The lyophilised product is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at 4°C, not refrozen, and preferably used the same day. |
Note | For research use only. |
Isotype | IgG1 |
Clonality | Monoclonal |
Application Notes | The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species. Conjugation Notes: A proprietary technique for the binding to TRITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence. Excitation: 554 nm, emission: 573 nm. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. |
产品资料 |