产品名称 Goat Serum Against Mouse IgG1 IgG2A IgG2B IgG3
产品货号 orb22160
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Goat Serum Against Mouse IgG1 IgG2A IgG2B IgG3
Catalog Number orb22160
Species/HostGoat
ConjugationUnconjugated
Tested applicationsDOT, IEP, IP
Immunogen Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Target IgG1, IgG2A, IgG2B, IgG3. The reactivity of the antiserum is directed to the Fc and Fab subunits of IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against mouse serum a single characteristic precipitin line with a spur is usually obtained. It does not react with any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Form/Appearance Vial with 1 ml lyophilized antiserum.
Concentration Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added.
Preservatives No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Storage The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Note For research use only.
ClonalityPolyclonal
Adsorption No adsorption required.
Application Notes IE: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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