| 产品名称 |
Rabbit Serum Against Mouse IgG1 IgG2A IgG2B IgG3 IgA IgM |
| 产品货号 |
orb22381 |
| 产品价格 |
现货询价，电话：010-67529703
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| 产品规格 |
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| 产品品牌 |
Biorbyt |
| 产品概述 |
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| 产品详情 |
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| Product Name | Rabbit Serum Against Mouse IgG1 IgG2A IgG2B IgG3 IgA IgM |
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| Catalog Number | orb22381 |
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| Species/Host | Rabbit |
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| Conjugation | Unconjugated |
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| Tested applications | DOT, IEP, IP |
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| Immunogen | Purified polyclonal IgG and pools of homogenous IgA and IgM isolated from mouse serum. Freunds complete adjuvant is used in the first step of the immunization procedure. |
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| Target | IgG1, IgG2A, IgG2B, IgG3, IgA, IgM. The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectro- phoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. |
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| Form/Appearance | Vial with 1 ml lyophilized antiserum. |
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| Concentration | Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added. |
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| Preservatives | No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
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| Storage | The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
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| Note | For research use only. |
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| Clonality | Polyclonal |
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| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity with other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
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| Application Notes | IE: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
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