产品名称 Rabbit Serum Against Swine IgG
产品货号 orb22414
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Rabbit Serum Against Swine IgG
Catalog Number orb22414
Species/HostRabbit
ConjugationUnconjugated
Tested applicationsDOT, IEP, IP
Immunogen Purified normal IgG isolated from pooled swine serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Target IgG. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in swine serum, as tested by immuno- electrophoresis and double radial immunodiffusion.
Form/Appearance Vial with 1 ml lyophilized antiserum.
Concentration Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added.
Preservatives No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Storage The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Note For research use only.
ClonalityPolyclonal
Adsorption No adsorption required.
Application Notes Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: bovine + cat + chicken - dog + duck - goat + guinea pig + human + horse + mouse + monkey + pigeon - rat + sheep + turkey -

IE: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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