产品名称 Human IL6 protein | Biorbyt
产品货号 orb80063
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Human IL6 protein
Catalog Number orb80063
ConjugationUnconjugated
Tested applicationsFunctional Assay, HPLC, SDS-PAGE
Target Human IL6
Alternative Names
Form/Appearance Lyophilized from 0.2 um filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Protein Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.
Purity > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
Biological Origin E.coli
Activity The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ng/ml, corresponding to the specific activity of 1.0 10,000,000 Units per mg.
Application Notes Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-6 as Reference Standard.
Solubility (25°C) It is recommended to reconstitute the lyophilized Interleukin-6 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
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