| 产品名称 |
Human TNF alpha protein | Biorbyt |
| 产品货号 |
orb80107 |
| 产品价格 |
现货询价，电话：010-67529703
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| 产品规格 |
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| 产品品牌 |
Biorbyt |
| 产品概述 |
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| 产品详情 |
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| Product Name | Human TNF alpha protein |
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| Catalog Number | orb80107 |
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| Conjugation | Unconjugated |
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| Tested applications | Functional Assay, HPLC, SDS-PAGE |
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| Target | Human TNF alpha |
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| Alternative Names | (click to expand) APC1 protein, Cachectin protein, DIF protein, Differentiation inducing factor protein, Macrophage cytotoxic factor protein, MCF protein, Necrosin protein, TNF a protein, TNF alpha protein, TNF protein, TNF Macrophage Derived protein, TNF Monocyte Derived protein, TNF-a protein, TNF-alpha protein, TNFA protein, Tumor necrosis factor alpha protein, TNF-alpha protein |
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| Form/Appearance | 1mg of TNF-alpha Human contain 20 mM PBS, pH-7.2, and 10mM NaCl. |
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| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
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| Note | For research use only. |
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| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Arg-Ser-Ser. |
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| Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
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| Biological Origin | E.coli |
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| Activity | The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D, corresponding to Specific Activity of 50,000,000 IU/mg. |
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| Application Notes | Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of TNF-a as Reference Standard. |
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| Solubility (25°C) | It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
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| 产品资料 |
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