产品名称 |
Mouse TNF alpha protein | Biorbyt |
产品货号 |
orb80109 |
产品价格 |
现货询价,电话:010-67529703
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产品规格 |
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产品品牌 |
Biorbyt |
产品概述 |
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产品详情 |
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Product Name | Mouse TNF alpha protein |
Catalog Number | orb80109 |
Conjugation | Unconjugated |
Tested applications | Functional Assay, HPLC, SDS-PAGE |
Target | Mouse TNF alpha |
Alternative Names | (click to expand) APC1 protein, Cachectin protein, DIF protein, Differentiation inducing factor protein, Macrophage cytotoxic factor protein, MCF protein, Necrosin protein, TNF a protein, TNF alpha protein, TNF protein, TNF Macrophage Derived protein, TNF Monocyte Derived protein, TNF-a protein, TNF-alpha protein, TNFA protein, Tumor necrosis factor alpha protein, TNF-alpha protein |
Form/Appearance | Lyophilized from 0.2 um filtered concentrated solution in PBS, pH 7.2. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Note | For research use only. |
Protein Sequence | MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
Purity | > 98.0% as determined by RP-HPLC and SDS-PAGE analysis |
Biological Origin | E.coli |
Activity | The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin is 0.1ng/ml, corresponding to Specific Activity of 10,000,000 Units/mg. |
Application Notes | Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.24 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of TNF-a as Reference Standard. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
产品资料 |
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