产品名称 Properdin antibody (FITC) | anti Properdin antibody (FITC) | Biorbyt
产品货号 orb21995
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Properdin antibody (FITC)
Catalog Number orb21995
Species/HostGoat
ReactivityHuman
ConjugationFITC
Tested applicationsDOT, IEP
Target Properdin (FITC). In immunoelectrophoresis against human serum a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma.
Form/Appearance Vial with 1 ml lyophilized immunoconjugate.
Concentration IgG protein concentration 1.7 mg/ml. Fluorescein/IgG protein molar ratio (F/P) approximately 1.3. No foreign proteins added.
Preservatives No preservative added, as it may interfere with the antibody activity.
Storage Lyophilized at 4°C is stable for at least 10 years. Reconstituted at or below -20°C is stable for at least 3-5 years. Reconstituted at 4°C is stable for at least 7 days.
Note For research use only.
ClonalityPolyclonal
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Uniprot ID P27918
Application Notes The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

IE: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C . It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C . Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not re frozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Conjugation Notes: A proprietary technique for the binding to FITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Fluorescein isothiocyanate isomer 1 (FITC). Excitation: 492 nm, emission : 515 nm.

Experiment Notes: Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
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