产品名称 Transferrin antibody | anti Transferrin antibody | Biorbyt
产品货号 orb21918
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Transferrin antibody
Catalog Number orb21918
Species/Host Goat
Reactivity Aves
Conjugation Unconjugated
Tested applications DOT, IEP
Immunogen Purified transferrin isolated from pooled duck serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Target Transferrin. The reactivity of the antiserum is restricted to transferrin as tested in immunoelectro- phoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin.
Form/Appearance Vial with 1 ml lyophilized antiserum.
Concentration Precipitin titre 1:32 when tested against pooled normal duck serum in agar-block immunodiffusion titration.
Preservatives No preservative added.
Storage The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refro zen, and preferably used the same day.
Note For research use only.
Clonality Polyclonal
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Purity wells.
Application Notes Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross- reactivity of this antiserum has not been tested in detail, however in immunoelectrophoresis a reaction has been observed with chicken and pigeon.

IE: In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 ulantiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
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