产品名称 | Porcine IL 1 beta protein | Biorbyt |
产品货号 | orb80409 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | |
产品品牌 | Biorbyt |
产品概述 | |
产品详情 | |
Product Name | Porcine IL 1 beta protein |
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Catalog Number | orb80409 |
Reactivity | Porcine |
Conjugation | Unconjugated |
Tested applications | SDS-PAGE |
Target | Porcine IL 1 beta |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. The IL 1 beta was lyophilized from a 0.2μm filtered concentrated solution in PBS pH 7.4, containing 3 % trehalose. |
Storage | Store at 4°C.for up to two weeks. For long term storage, aliquot and store at -20°C. |
Note | For research use only. |
Protein Sequence | ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP |
Purity | >95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Source | E coli |
Activity | The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 |
Application Notes | Experiment Notes: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-1b as Reference Standard. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IL 1 beta in sterile distilled H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
产品资料 |