产品名称 Porcine IL 1 beta protein | Biorbyt
产品货号 orb80409
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Porcine IL 1 beta protein
Catalog Number orb80409
ReactivityPorcine
ConjugationUnconjugated
Tested applicationsSDS-PAGE
Target Porcine IL 1 beta
Form/Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
The IL 1 beta was lyophilized from a 0.2μm filtered concentrated solution in PBS pH 7.4, containing 3 % trehalose.
Storage Store at 4°C.for up to two weeks. For long term storage, aliquot and store at -20°C.
Note For research use only.
Protein Sequence ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP
Purity >95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Source E coli
Activity The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0
Application Notes Experiment Notes: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-1b as Reference Standard.
Solubility (25°C) It is recommended to reconstitute the lyophilized IL 1 beta in sterile distilled H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
产品资料