产品名称 Mouse CSF1R Human Cells Transfected Lysate (positive control) (denatured) | Biorbyt
产品货号 orb174403
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Mouse CSF1R Human Cells Transfected Lysate (positive control) (denatured)
Catalog Number orb174403
Hazard Information Non-Toxic
Target Mouse CSF1R
Form/Appearance 50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue
Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing
Note For research use only.
Biological Origin Human Cells
Application Notes Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol
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