| 产品名称 | Rat CD133 Human Cells Transfected Lysate (positive control) (denatured) | Biorbyt |
| 产品货号 | orb174679 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Rat CD133 Human Cells Transfected Lysate (positive control) (denatured) |
|---|---|
| Catalog Number | orb174679 |
| Hazard Information | Non-Toxic |
| Target | Rat CD133 |
| Form/Appearance | 50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue |
| Storage | Store at -80°C. Aliquot to avoid repeated freezing and thawing |
| Note | For research use only. |
| Biological Origin | Human Cells |
| Application Notes | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol |
| 产品资料 |