| Application Notes |
Experiment Notes: - Capture Antibody: 1.0 mg/mL of mouse anti-E. coli STX2B monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating.
- Detection Antibody: 0.25 mg/mL mouse anti-E. coli STX2B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 μg/mL in Detection Antibody dilution buffer before use.
- Standard: Each vial contains 19 ng of recombinant E. coli STX2B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
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