| 产品名称 | TNF-alpha antibody | anti TNF-alpha antibody | Biorbyt |
| 产品货号 | orb303922 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | TNF-alpha antibody |
|---|---|
| Catalog Number | orb303922 |
| Species/Host | Goat |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, S-ELISA, WB |
| Immunogen | Produced from sera of goats pre-immunized with highly pure recombinant Human TNF-alpha |
| Target | TNF-alpha |
| Form/Appearance | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Concentration | 0.1-1.0 mg/ml |
| Storage | Store at 4°C for short term and at-20°C for long term storage. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by affinity chromatography |
| Uniprot ID | P01375 (Human) |
| Dilution Range | WB: 0.1-0.2 μg/ml, IHC-P: 2.5 μg/ml-5.0 μg/ml |
| Application Notes | WB Notes: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. ELISA Notes: To detect Human TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 2.5 μg/ml-5.0 μg/ml with a two-hour incubation at room temperature or an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. |
| 产品资料 |