产品名称 IgM antibody | Biorbyt
产品货号 orb108288
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name IgM antibody
Catalog Number orb108288
Species/HostGoat
ConjugationUnconjugated
Tested applicationsELISA, IHC-Fr, WB
Target IgM. Minimal cross reaction to Bovine Serum Proteins. Based on immunoelectrophoresis, the antibody reactswith the Fc5u portion of the human IgM heavy chain. No antibody was detected against normal human IgGor IgA, or against non immunoglobulin serum proteins. Has been tested by ELISA and or solid phaseadsorbed to ensure minimal cross reaction with bovine serum proteins, but may cross react with IgM fromother species.
Form/Appearance Lyophilized from 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 containing 15mg/ml BSA
Concentration 0.8mg/ml (prior to lyophilization)
Preservatives No Preservative.
Storage Store lyophilized product at 2-8°C. After reconstitution, store for several weeks at 2-8°C as an undiluted liquid. Prepare working dilution only prior to immediate use. Long term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles (which may occur in "frost free" freezers.) Expiration date is one year from date of reconstitution.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified
Biological Origin Sample obtained from Goat.
Dilution Range IHC-Fr:1:500-1:5,000, ELISA:1:5,000-1:100,000, WB:1:10,000-1:200,000
Application Notes A dilution range of 1:500-1:5,000 is suggested for immunohistochemistry on tissue sections, 1:5,000 -1:100,000 for ELISA and Western blotting with chromogenic substrates and 1:10,000-1:200,00 for Westernblotting with ECL substrates.Each laboratory should determine an optimum working titer for use in itsparticular application.Other applications have not been tested but use in such assays should not necessarilybe excluded.Use of sodium azide as a preservative will substantially inhibit the enzyme activity ofhorseradish peroxidase.Centrifuge before opening to ensure complete recovery of vial contents.
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