产品名称 |
Human VEGF protein | Biorbyt |
产品货号 |
orb80115 |
产品价格 |
现货询价,电话:010-67529703
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产品规格 |
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产品品牌 |
Biorbyt |
产品概述 |
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产品详情 |
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Product Name | Human VEGF protein |
Catalog Number | orb80115 |
Conjugation | Unconjugated |
Tested applications | Functional Assay, HPLC, SDS-PAGE |
Target | Human VEGF |
Alternative Names | (click to expand) Vascular endothelial growth factor A protein, Vascular Endothelial Growth Factor protein, Vascular Permeability Factor protein, VEGF-A protein, VEGFA protein, VPF protein |
Form/Appearance | The protein was lyophilized from concentrated (1mg/ml) solution with no additives. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Note | For research use only. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Ala-Glu. |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Biological Origin | E.coli |
Activity | Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using concentration range of 1.0-8.0 ng/ml. |
Application Notes | Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of VEGF as Reference Standard. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
产品资料 |
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