产品名称 Human VEGF protein | Biorbyt
产品货号 orb80115
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Human VEGF protein
Catalog Number orb80115
ConjugationUnconjugated
Tested applicationsFunctional Assay, HPLC, SDS-PAGE
Target Human VEGF
Alternative Names
Form/Appearance The protein was lyophilized from concentrated (1mg/ml) solution with no additives.
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Protein Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Ala-Glu.
Purity > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
Biological Origin E.coli
Activity Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using concentration range of 1.0-8.0 ng/ml.
Application Notes Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of VEGF as Reference Standard.
Solubility (25°C) It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
产品资料