| 产品名称 | Rabbit Serum Against Mouse IgD |
| 产品货号 | orb22375 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Rabbit Serum Against Mouse IgD |
|---|---|
| Catalog Number | orb22375 |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Tested applications | DOT, IEP |
| Immunogen | Highly purified polyclonal and monoclonal IgD isolated from Balb/C and C57Bl serum. Freunds complete adjuvant is used in the first step of the immunization procedure. |
| Target | IgD. The reactivity of the antiserum is restricted to the Fc portion of the IgD molecule, which represents strict isotype (class specificity. In immunoelectrophoresis and radial immunodif- fusion, using various antiserum concentrations against mouse serum belonging to different allotypic groups, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with the purified IgD from Balb/C and C57Bl. No precipitation reaction is obtained with purified IgG, including all subclasses, IgA, IgM, IgG/Fab fragments or other serum proteins. The antiserum also reacts with membrane- bound IgD in peripheral blood cells of different mouse strains as tested by immuno- fluorescence microscopy. |
| Form/Appearance | Vial with 1 ml lyophilized antiserum. |
| Concentration | Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. |
| Preservatives | No preservative added. |
| Storage | Lyophilized at 4°C is stable for at least 10 years. Reconstituted at or below -20°C is stable for at least 3-5 years. Reconstituted at 4°C is stable for at least 7 days. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the elimination of antibodies to he common Ig/Fab portion. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application Notes | The antiserum does not cross-react with any other component of the mouse Ig system. Inter-species cross reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants, but this has not been tested because of the very low level of IgD in most species. Experiment Notes: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C . Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. |
| 产品资料 |