产品名称 TNF-alpha antibody | anti TNF-alpha antibody | Biorbyt
产品货号 orb303923
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name TNF-alpha antibody
Catalog Number orb303923
Species/HostRabbit
ReactivityMouse
ConjugationUnconjugated
Tested applicationsIHC-P, S-ELISA, WB
Immunogen Produced from sera of rabbits pre-immunized with highly pure recombinant Murine TNF-alpha
Target TNF-alpha
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Purified by affinity chromatography
Uniprot ID P06804 (Mouse)
Dilution Range WB: 0.1-0.2 μg/ml, IHC-P: 1.0 μg/ml
Application Notes WB Notes: To detect Murine TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine TNF-α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Notes: To detect Murine TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
IHC Notes: This antibody stained colchicine injected mouse brain (hippocampus CA1 region) tissue.  The primary antibody was incubated at 1.0 μg/ml overnight at 4°C.  This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent.  Optimal concentrations and conditions may vary.
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