产品名称 | TNF-alpha antibody | anti TNF-alpha antibody | Biorbyt |
产品货号 | orb303923 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | |
产品品牌 | Biorbyt |
产品概述 | |
产品详情 | |
Product Name | TNF-alpha antibody |
---|---|
Catalog Number | orb303923 |
Species/Host | Rabbit |
Reactivity | Mouse |
Conjugation | Unconjugated |
Tested applications | IHC-P, S-ELISA, WB |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure recombinant Murine TNF-alpha |
Target | TNF-alpha |
Form/Appearance | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Concentration | 0.1-1.0 mg/ml |
Storage | Store at 4°C for short term and at-20°C for long term storage. |
Note | For research use only. |
Isotype | IgG |
Clonality | Polyclonal |
Purity | Purified by affinity chromatography |
Uniprot ID | P06804 (Mouse) |
Dilution Range | WB: 0.1-0.2 μg/ml, IHC-P: 1.0 μg/ml |
Application Notes | WB Notes: To detect Murine TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine TNF-α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. ELISA Notes: To detect Murine TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. IHC Notes: This antibody stained colchicine injected mouse brain (hippocampus CA1 region) tissue. The primary antibody was incubated at 1.0 μg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary. |
产品资料 |