| 产品名称 | Secretory IgA antibody |
| 产品货号 | orb21244 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Secretory IgA antibody |
|---|---|
| Catalog Number | orb21244 |
| Species/Host | Sheep |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | DOT, IEP |
| Immunogen | Highly purified secretory IgA isolated from pooled human milk. Freunds complete adjuvant is used in the first step of the immunization procedure. |
| Target | Secretory IgA. The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and to surface and hidden determinants of the secretory component as tested in immunoelectrophoresis and radial immunodiffusion. In immunoelectrophoresis using various antiserum concentrations against normal human milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal human serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. |
| Form/Appearance | Vial with 1 ml lyophilized antiserum. |
| Concentration | Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added. |
| Preservatives | No preservative added. |
| Storage | The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross- reacting with other components of the immunoglobulin system or reacting with other serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application Notes | The antiserum does not cross-react with any other component of the human Ig system. Inter- species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. IE: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| 产品资料 |